Expression Database

Data Source

Gene

Papss2

3'-phosphoadenosine 5'-phosphosulfate synthase 2

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros through pelvis and proximal ureter
	Cortex, medulla - expression in upper limb of comma-shaped bodies and renal tubules
	Nephrogenic zone cortex - note expression in pretubular aggregates and upper limb of comma-shaped bodies
	Metanephros through cortex, medulla and pelvis
	Cortex - expression in early tubule and medial S-shaped body
	Cortex - expression in pretubular aggregates or renal vesicles, upper limb of comma-shaped body and early tubules
	Cortex - expression in distal end of renal vesicles
	Cortex - note strong expression in distal renal vesicle and upper limb comma-shaped bodies
	Metanephroi

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	97
Submission ID:	ml_170108_6751

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Papss2

Name: 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (MGI:1330223)

Probe ID:

MGI:1907296 (maprobe:4259)

Name of cDNA:

RIKEN clone 1810018P12

Sequence ID:

AK007539.1

5' primer sequence:

CCATCCTCCTAAGCTAGGGC

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGGCTACAGCAGAAAGGCACT

5' primer location:

262

3' primer location:

828

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1358

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7um sections 12 sections examined including metanephros and proximal ureter. Experiment: SISH010807 Slides A12&A13 1358 Colour development for 75.2hrs. Control probes Wnt4, Wnt7b and Shh on TS23 metanephros colour developed for 69hrs, 69hrs and 120hrs respectively. Imaging: Manualscan06-08-07 Image_.vsi.

Linked Publications

Linked Submissions

Acknowledgements