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Overview of Prostate Development | GenitoUrinary Development Molecular Anatomy Project (GUDMAP)
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Overview of Prostate Development

Provided by Chad Vezina, University of Wisconsin-Madison

All male mammals (even whales) have a prostate. Current knowledge of prostate development is largely based on observations in rodents and this tutorial focuses on mouse prostate development.

The urogenital sinus gives rise to the prostate

The prostate derives from a transient fetal structure known as the urogenital sinus, or UGS for short. The UGS is an enlarged portion of pelvic urethral positioned between bladder and penile urethra.

UGS and other components of a 16 dpc male mouse lower urinary tract - whole mount
This image shows the UGS and other components of a 16 dpc (days post conception) male mouse lower urinary tract.
UGS and other components of a 16 dpc male mouse lower urinary tract - saggital
This image is a hematoxylin and eosin-stained UGS tissue section.

The UGS consists of several cell types which are generally grouped into an inner layer of UGS epithelium and an outer later of UGS mesenchyme. The section is located near the mid saggital plan and the pelvic urethral lumen is evident in the middle of the section as depicted in the above right image.

Androgens initiate prostate development by patterning gene expression in UGS epithelium and stroma

Prostate development begins after testicular testosterone synthesis initiates at 13 days post coitus in mice and gestation week 10 in humans.

The androgen receptor plays a key role in prostate development. It is expressed by male and female UGS epithelial and mesenchyme cells, as shown by the immunostained images on the left of the image below. Androgen receptor immunostaining appears in red. A yellow dashed line indicates the boundary between the inner layer of UGS epithelium and outer layer of prostate mesenchyme.

Androgen receptor activation is necessary for prostate development based on observations that prostate does not form in castrated rodents or in mice harboring an inactive form of the androgen receptor. Androgen receptor activation is also sufficient for prostate development based on observations that androgen exposure drives prostate formation in female rodent fetuses.

The UGS is initially ambisexual, but in response to testicular androgens undergoes morphological and molecular changes. Some of these include:

  • steroid 5 alpha reductase type 2 expression by male UGS stroma (shown in middle images)
  • NK3 homeobox 1 expression by UGS epithelium (shown in right images).
  • UGS shortening and an acute angle change between the bladder neck and caudal pelvic urethra (note the angle difference between male and female UGS as shown in the left images)

Most if not all molecular and morphological changes occurring during male UGS development are driven by androgen receptor activation in UGS stroma.

Prostate development requires functional AR in UGS mesenchyme, not in UGS epithelium

A clever set of experiments by Drs. Ilse Lasnitzki and Takeo Mizuno demonstrated that androgen receptors in UGS mesenchyme are needed for prostate development while those in UGS epithelium are not needed.

They collected UGSs from control mice and from mice harboring a spontaneous missense mutation in the androgen receptor that introduces a premature stop codon and is translated into a non-functional truncated protein. Using digestive enzymes and a steady set of hands, they carefully dissected the UGS mesenchyme (or UGM) away from UGS epithelium (or UGE), and then recombined the tissues in various ways before incubating them in organ culture media containing testosterone.

As a positive control, when wild type UGS mesenchyme and epithelium were recombined, they grew prostatic buds, which are the precursors of prostate ducts and discussed in detail in the next section.

As a negative control, when androgen receptor mutant UGS mesenchyme and epithelium were recombined, they failed to form prostatic buds. In fact, the only ways prostatic buds would form was if the source of UGS mesenchyme used in these experiments contained a functional androgen receptor.

Thus, functional androgen receptors in UGS mesenchyme are required for prostate development.

UGS mesenchyme is an instructive (but not permissive) inducer of prostate development

The formation of most organs requires the action of one group of cells changing the behavior of a nearby group of cells in a process known as induction. It was clear from the work of Lasnitzki and Mizuno that the prostate is induced by androgen receptors in the UGS mesenchyme. But it was not clear how this occurred.

Howard Holtzer (1968) described two modes of induction when considering formation of bone: permissive and instructive induction.

Permissive induction

In permissive induction, the responding tissue already possesses all of the information needed to reach its potential. In other words, it knows what it wants to be when it grows up, it is just waiting for permission. This type of inductive interaction is illustrated for the prostate in panel 1.

Illustration of permissive induction

If the prostate was permissively induced, some UGS epithelial cells would already know they were fated to become prostate epithelium. These cells are indicated by yellow shading. Testicular androgens would bind and activate UGS mesenchymal androgen receptors, yielding a signal that would be sensed by the UGS epithelial cells, and prostate development would begin.

Instructive induction

In instructive induction, the responding tissue does not possess the information needed to reach its potential (it does not know what to become when it grows up). The identity crisis of the UGS epithelium is signified by the absence of yellow staining in the above image. In response to testicular androgens, the UGS mesenchyme would synthesize and disseminate instructions to the underlying epithelium, informing it to become a prostate and undergo ductal development.

Dr. Gerald Cunha and colleagues demonstrated that the UGS stroma is an inductive inducer of prostate development by performing tissue recombination experiments using fetal mouse UGS and adult mouse bladder. The epithelial and mesenchymal components of both tissues were separated and recombined similar to the methods used in the Lasnitzki and Mizuno experiment described above, and then the tissues were implanted under the capsule of the kidney of male mice, where they vascularized and matured. UGS mesenchyme was shown to reprogram bladder epithelium into a glandular prostatic epithelium, demonstrating that it is instructive.

Table of tissue formed due to mesenchyme or epithelial source

Prostate morphogenesis

The prostate undergoes several key developmental milestones before forming the glandular network of the mature prostate.

It initially comprises a relatively simple tube of endoderm-derived UGS epithelium surrounded by mesoderm-derived UGS mesenchyme.

During the induction phase, androgens activate androgen receptors in the UGS mesenchyme.

During the specification phase, UGS mesenchyme cells instructively program underlying epithelial cells and the specified epithelial cells express Nkx3-1.

Movie of budding and branching phases

During the budding phase, solid cords of UGS epithelium grow into UGS mesenchyme in a bilaterally symmetrical and stereotypical pattern. Mature mouse and rat prostate consist of 4 bilaterally symmetrical lobes (the anterior, dorsal, lateral, and ventral lobes) and the anatomical position of these lobes is determined by the anatomical pattern of anterior, dorsal, lateral, and ventral prostatic buds.

During the branching phase, prostatic buds elongate and successively branch (the pattern is stereotypical for each prostatic lobe).

Prostatic ducts form lumens that give rise to the mature prostate.

A movie depicting the budding and branching phases is shown below.

The prostatic buds are color coded:

  • Red buds are anterior buds that will form the anterior prostate lobes.
  • Green buds are dorsal and lateral buds and will form dorsal and lateral prostate lobes.
  • Blue buds are ventral prostate buds and will form ventral prostate lobes.

The following illustrations are cartoons of the gross appearance of the lower urinary tract during budding and branching morphogenesis.

Gross eppearance of the LUT during morphogenesis

Below are schematic diagrams of the multi-layered cells comprising the 17 dpc male mouse UGS and the P50 (Postnatal, ie 50 days after birth) mouse prostate.

Histological eppearance of 17 dpc male mouse UGS and the P50 mouse prostate

 

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