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Tissue Hub | GenitoUrinary Development Molecular Anatomy Project (GUDMAP)

Tissue Hub

Rajiv Dhir (PI)
University of Pittsburgh

  • Stefan Kostadinov
    University of Pittsburgh
  • Sunder Sims-Lucas
    University of Pittsburgh
  • Susan Kelly
    University of Pittsburgh

Project Description

We have preliminary data that we can isolate human genitourinary tissues (kidneys, ureters and bladders) from various developmental ages (6-24 weeks). We have produced publication quality histological images of the developing urogenital organs (including kidneys and bladder), and have immunostained kidneys for endothelium, nephron progenitors and early-differentiated nephron structures and bladders for the urothelium and muscle layers. We have utilized Dynabeads® to separate distinct cellular subpopulations in the kidney including: nephron progenitors, ureteric epithelium, podocytes and endothelium, and have confirmed that we can produce high quality material that is appropriate for RNA sequencing. We propose to act as both the GUDMAP Tissue Hub and Tissue Gathering site to build upon the pre-existing HSTB and provide top quality genitourinary samples to members of the scientific community including those within GUDMAP.