Expression Database

GUDMAP:2206
 
Theiler Stage TS26
   
Supplemental Data Files
CEL file: KPL_gd18_6_epididymus_Mouse430_2_KWGU011601_b.CEL
CHP file: KPL_gd18_6_epididymus_Mouse430_2_KWGU011601_b.CHP
RPT file: KPL_gd18_6_epididymus_Mouse430_2_KWGU011601_b.RPT
TXT file: KPL_gd18_6_epididymus_Mouse430_2_KWGU011601_b.TXT
   
Archive ID 27
   
Principal Investigator(s) Kevin Gaido, The Hamner Institutes of Health Sciences, Durham, NC, USA, gaido@thehamner.org
   
Submitted By Kim Lehmann, The Hamner Institutes of Health Sciences, Durham, NC, USA, klehmann@thehamner.org
   
Specimen Details
Sample GEO ID: GSM120827
Sample Description: gd 18 whole epididymis
Title: Gaido_Epididymis_gd18_6_25May2006
Component(s) Sampled: epididymis (EMAP:13166)
Organism: Mus musculus
Strain: CD-1
Genotype: Wild type
Sex: Male
Development Age: 18 dpc
Theiler Stage: 26
Pool Size: NA
Pooled Sample: No
Dissection Method: Whole organ excision
Experimental Design: CD-1 mice are time-mated within a 2 hour window. Pregnant CD-1 mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal epididymides are removed from fetuses at gestation days 12, 14, 16, 18, and postnatal day 2, snap frozen in liquid nitrogen and stored at -80ºC.
   
Array Hybridization
Extracted Molecule: total RNA
A260:280 Ratio: 1.89
RNA Extraction Protocol: Gaido protocol: 'RNA isolation from whole testes'
Target Amplification Manufacturer/kit: Affymetrix
Target Amplification Protocol: Affymetrix Two-Cycle cDNA synthesis protocol (701025 Rev 5)
Rounds of Amplification: 2
Amount Labeled Target Hybridized To Array: 15 ug
Label: Biotin
Label Protocol:
Array Hyb/Wash Protocol: Affymetrix standard protocol (701021 Rev 4)
Scan Protocol:
GCOS Tgt Value: 500
Data Analysis Method: Affymetrix GCOS and SAS Microarray Solutions
Reference: P0.5 whole embryo RNA (4 male/4 female) provided by Steve Potter
   
Series Details
Series GEO ID: GSE5333
Number of Samples: 16 samples
Title: Gene expression profiling of whole epididymis in embryonic (gestational day 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 4)
Summary: The overall objective of this proposal is to map the temporal and spatial dynamics of gene expression in the fetal mouse testis at key developmental timepoints. Urogenital tract malformations are the most common birth defects in males and their incidence together with other male reproductive health concerns such as reduced fertility and testicular cancer are reportedly on the rise in the human population. To better understand the impact of genetic factors and environmental influences on testicular development, it is important to first understand normal gene expression patterns and signaling cascades within the fetal testis during development. The goal of this study is to identify cell-specific genes that can be used as biomarkers for key differentiation events.
Type: developmental time course
Overall Design: Day to day comparison in whole epididymis throughout development with triplicates at each time point. Each sample is a pair of epididymis from a single animal and each animal is taken from a different dam.
   
Platform Details
Platform GEO ID: GPL1261
Title: Affymetrix GeneChip Mouse Genome 430 2.0 Array
Distribution: commercial
Technology: in situ oligonucleotide
Organism: Mus musculus
Manufacturer: Affymetrix
Manufacturer Protocol: N/A
Catalogue Number: N/A
   

 

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Department of Health and Human Services National Institutes of Health Contacts Citing GUDMAP National Institute of Diabetes and National Institute of Child Health and Human Development