Day to day comparison in whole testes throughout development with triplicates at each time point. Each sample is a pair of testes from a single animal and each animal is taken from a different dam.
Array Hybridization
Molecule:
total RNA
A_260_280:
2.12
RNA Extraction Protocol:
Gaido protocol: 'RNA isolation from whole testes'
Target Amplification Manufacturer/kit:
NA
Target Amplification Protocol:
NA
Rounds of Amplification:
NA
Amount Labeled Target Hybridized To Array:
10 ug
Label:
Biotin
Array Hyb/Wash Protocol:
Affymetrix standard protocol (701021 Rev 4)
GCOS Tgt Value:
500
Data Analysis Method:
Affymetrix GCOS and SAS Microarray Solutions
Reference:
P0.5 whole embryo RNA (4 male/4 female) provided by Steve Potter
Gene expression in whole testes at gd 11, 12, 14, 16, 18 and pnd2
Summary:
The overall objective of this proposal is to map the temporal and spatial dynamics of gene expression in the fetal mouse testis at key developmental timepoints. Urogenital tract malformations are the most common birth defects in males and their incidence together with other male reproductive health concerns such as reduced fertility and testicular cancer are reportedly on the rise in the human population. To better understand the impact of genetic factors and environmental influences on testicular development, it is important to first understand normal gene expression patterns and signaling cascades within the fetal testis during development. The goal of this study is to identify cell-specific genes that can be used as biomarkers for key differentiation events.
Type:
developmental time course
Overall Design:
Day to day comparison in whole testes throughout development with triplicates at each time point. Each sample is a pair of testes from a single animal and each animal is taken from a different dam.
