Expression Database

GUDMAP:9161

Data Source

Gene

Mettl7b

methyltransferase like 7B

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros
	Nephrogenic zone and cortex - expression absent from early nephrons and capillary loop nephrons and detected in a subset of cortical renal tubules of maturing nephrons
	Cortex
	Metanephros

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	101
Submission ID:	ml_180108_6794

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Mettl7b

Name: methyltransferase like 7B (MGI:1918914)

Probe ID:

MGI:1904242 (maprobe:4774)

Name of cDNA:

RIKEN clone 0610006F02

Sequence ID:

AK002239.1

5' primer sequence:

TTCCAGTTCTACCCACAGGG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCAAAGTCCAGGGCTTAGCAG

5' primer location:

304

3' primer location:

975

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1870

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7um sections 10 sections examined including metanephros and proximal ureter. Experiment: SISH231007 1870 Robot SISH Slide A2 Colour development for 100hrs. Control probes Wnt4 and Shh on TS23 metanephros colour developed for 49hrs and 100hrs respectively. Imaging: Manual Scan 09-11-07 Image_8.vsi. Probe derived from early proximal tubule TS23 subcompartment UQ Method 1 gene list. Note that the medulla was not examined as it was not included in these sections.

Linked Publications

Linked Submissions

Acknowledgements