Expression Database

GUDMAP:9136

Data Source

Gene

Sord

sorbitol dehydrogenase

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros sectioned through pelvis
	Cortex and medulla - expression in early proximal tubules
	Metanephros sectioned through pelvis and proximal ureter
	Nephrogenic zone and cortex
	Nephrogenic zone through to pelvis (top)
	Cortex
	Cortex - note expression absent from first segment of early proximal tubule at urinary pole of maturing renal corpuscle (centre right)

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	101
Submission ID:	ml_180108_6769

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Sord

Name: sorbitol dehydrogenase (MGI:98266)

Probe ID:

MGI:1897762 (maprobe:4765)

Name of cDNA:

RIKEN clone 4930403C20

Sequence ID:

AK015059.1

5' primer sequence:

CTGCCGATTCTACAAGCACA

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCAAAGGCTTCAACTGCCTTC

5' primer location:

442

3' primer location:

1061

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1928

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7um sections 10 sections examined including metanephros and proximal ureter. Experiment: SISH051207 1928 Robot SISH Slide A8 Colour development for 28hrs. Control probes Wnt4 and Shh on TS23 metanephros colour developed for 120hrs and 120hrs respectively. Imaging: Manual Scan 13-12-07 Image_7.vsi. Probe derived from early proximal tubule TS23 subcompartment UQ Method 1 gene list.

Linked Publications

Linked Submissions

Acknowledgements