Expression Database

Data Source

Gene

Gpd1

glycerol-3-phosphate dehydrogenase 1 (soluble)

Theiler Stage

TS28

Tissue

metanephros

Images

	Metanephros transverse sections through inner medulla and proximal ureter.
	Cortex and outer medulla (top) - signal in renal proximal convoluted tubule of cortex.
	Cortex - signal detected in renal proximal convoluted tubule.
	Outer stripe of outer medulla - signal absent from proximal straight tubule.
	Inner stripe of outer medulla.
	Inner medulla.
	Metanephros section through inner medulla, pelvis and proximal ureter surrounded by cortex.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	100
Submission ID:	ml_290108_6928

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Gpd1

Name: glycerol-3-phosphate dehydrogenase 1 (soluble) (MGI:95679)

Probe ID:

MGI:1896828 (maprobe:4741)

Name of cDNA:

RIKEN clone 1200003G08

Sequence ID:

AK004565.1

5' primer sequence:

TGTGACCTTTCCCAGCTTTC

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGGCCTGACAGAAGACCTCAG

5' primer location:

1752

3' primer location:

2755

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1760

Specimen

Theiler Stage:

TS28

Other Staging System:

Adult sexually mature

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

female

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7um sections 4 sections examined including metanephros and proximal ureter. Experiment: SISH230707 Manual ISH SlideA14 1760 Colour development for 95hrs. Control probes Wnt4, Wnt7b and Shh on TS23 metanephros colour developed for 47hrs, 47hrs and 120hrs respectively. Imaging: Manualscan30-07-07 Image_8.vsi. Probe derived from early proximal tubule TS23 subcompartment UQ Method 1 gene list.

Linked Publications

Linked Submissions

Acknowledgements