Expression Database

GUDMAP:8200

Gene:	Wt1, Wilms tumor 1 homolog
Stage:	Theiler Stage 23
Tissue:	metanephros,ureter
Species:	Mus musculus
Assay Type:	IHC section

Images

	Metanephroi with proximal ureter. Wt1 protein visualised with DAB in orange and Wnt4 RNA visualised with BMpurple in blue.
	Metanephros. Note Wt1 protein in subset of early proximal tubules and subset of cortical and medullary interstitium adjacent to collecting ducts.
	Cortex & nephrogenic zone. Various nephron stages are represented. Wt1 protein in lower limb and Wnt4 RNA primarily in upper limb of comma- and S-shaped bodies.
	Nephrogenic zone. S-shaped body centre and renal vesicles.
	Nephrogenic zone.
	Maturing and capillary loop renal corpuslces and renal vesicle at right.
	Nephrogenic zone. S-shaped body and renal vesicles. Wt1 protein in the visceral and parietal epithelium of the proximal segement of the S-shaped body.
	Nephrogenic zone. A subset of the S-shaped body at the border between the proximal and medial segments contains both Wnt4 and Wt1 positive cells. There is no expression in the distal segment.
	S-shaped body connected to ureteric tip. No expression detected in the renal connecting tubule.
	Medulla and pelvis. Wt1 protein detected in a subset of medullary interstitium adjacent to collecting ducts. Some of these cells overlap with those that express Wnt4 RNA. The Wnt4 pattern appears to be a larger subset of interstitial cells.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Contributors:	Han Sheng Chiu, Bree Rumballe, Kylie Georgas
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Batch ID:	60
Submission ID:	ml_280507_6220

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Antibody

Protein:

Symbol: Wt1

Name: Wilms tumor 1 homolog (MGI:98968)

Name:

anti-Wt1

Accession ID:

MGI:3038122 (maprobe:4427)

Epitope (Uniprot ID):

P19544

Amino-terminus (start):

Carboxy-terminus (end):

181

Supplier:

DakoCytomation

Catalogue Number:

6F-H2

Lot Number:

Type:

Monoclonal

Host:

Hybridoma mouse clone 6F-H2

Purification Method:

tissue culture supernatant containing fetal bovine serum dialysed against 0.05mol/L Tris-HCl pH7.2 and 0.015mol/L sodium azide

Immunoglobulin Isotype:

IgG1

Variant Detected:

all isoforms of the full length human WT1 & WT1 minus exon 2 & in vitro translated WT1 & full length denatured WT1 55kD

Species Specificity:

mouse, human

Curator Notes:

Antigen=WT1 human. SwissProt accession number P19544.

Detection System:

Direct Label:

Secondary Antibody: anti-rabbit IgG (H+L), HRP conjugated antibody (Promega W4021)

Signal Detection Method: DAB (DAB Peroxidase Substrate Kit, Vector Laboratories, SK 4100) (brown-orange)

Detection Notes:

Dilution:

Notes:

Antigen=WT1 human. SwissProt accession number P19544.

Lab Antibody ID:

anti-Wt1

Specimen

Stage:

TS23

Other Staging System:

15.5 dpc

Tissue:

metanephros,ureter

Strain:

CD1

Genotype:

wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	Paraffin

Experiment Notes:

20 metanephros 7um sections including proximal ureter. Immunohistochemistry with anti-Wt1 antibody by DakoCytomation catalogue number 6F-H2 and visualised with DAB which produces orange signal. Experiment date: IHC260106 Imaging: Manualscan180906_16&17.vsi

Linked Publications

Kylie Georgas, Bree Rumballe, Lorine Wilkinson, Han Sheng Chiu, Emmanuelle Lesieur, Thierry Gilbert, Melissa H. Little
Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney
Histochem Cell Biol, 2008, 130(5):927-42
PubMed: 18618131

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:8209	same embryo same experiment same section