Expression Database

Data Source

Gene

Kcnj1

potassium inwardly-rectifying channel, subfamily J, member 1

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros sectioned through medulla with ureter.
	Nephrogenic zone and cortex.
	Medulla and cortex.
	Metanephros sectioned through cortex.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Divya Ramanth, Kylie Georgas, Melissa Little, Dave Tang, Han Sheng Chiu, Bree Rumballe, Sean Grimmond, Rathi Thiagarajan
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	253
Submission ID:	9999992575

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Kcnj1

Name: potassium inwardly-rectifying channel, subfamily J, member 1 (MGI:1927248)

Probe ID:

MGI:2422236 (maprobe:8689)

Name of cDNA:

RIKEN clone D630001P12

Sequence ID:

AK085262.1

5' primer sequence:

GCGGGAAGACTCTGGTTAAA

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCATCCTGACTGTGGTGCTGT

5' primer location:

2286

3' primer location:

2787

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2722

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7µm sections examined including 10 kidneys with ureters. Experiment: SISH010609 Probe ID 2722 Slide B3. Probe (1418613_at) identified from microarray screen of Potter group microarray data for genes enriched in the loop of Henle. Imaging: Batch Scan 12-06-09 Image_11.vsi.

Linked Publications

Thiagarajan RD, Georgas KM, Rumballe BA, Lesieur E, Chiu HS, Taylor D, Tang DT, Grimmond SM, Little MH. (2011) Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways Plos One, 6, e17286

Linked Submissions

Acknowledgements