Expression Database

Data Source

Gene

Slc22a6

solute carrier family 22 (organic anion transporter), member 6

Theiler Stage

TS28

Tissue

metanephros

Images

	Metanephros sectioned through inner medulla and pelvis.
	Renal cortex.
	Renal cortex and outer stripe of outer medulla.
	Renal cortex - expression in a subset of proximal convoluted tubule.
	Outer stripe of outer medulla.
	Inner stripe of outer medulla.
	Inner medulla and pelvis.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Sean Grimmond, Dave Tang, Rathi Thiagarajan, Kylie Georgas, Melissa Little, Han Sheng Chiu, Divya Ramanth, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	230
Submission ID:	9999991900

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Slc22a6

Name: solute carrier family 22 (organic anion transporter), member 6 (MGI:892001)

Probe ID:

MGI:2400327 (maprobe:4755)

Name of cDNA:

RIKEN clone 9630022J22

Sequence ID:

AK035971.1

5' primer sequence:

AGCACTAAAGGGAGTGGGGT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGAGCAACGTTGAAAACCCAT

5' primer location:

1777

3' primer location:

2355

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1962

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS28

Other Staging System:

Adult sexually mature

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7µm sections 4 sections examined including metanephros. Experiment: SISH281107 1962 SISH Slide B15. Gene identified as early proximal tubule specific in the developing kidney via microarray analysis of Brunskill et al, 2009 microarray data performed by Rathi Thiagarajan. Imaging: Batch Scan 04-12-07 Image_26.vsi. Colour development: This gene was colour developed for 120hrs. Control probe Wnt4 on TS23 metanephros colour developed for 29hrs. Shh weak expressing control probe colour developed for 120hrs. Moderate Shh expression signal was detected therefore low levels of mRNA expression should be detectable in this experiment.

Linked Publications

Linked Submissions

Acknowledgements