Expression Database

Data Source

Gene

Fbp2

fructose bisphosphatase 2

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros sectioned through pelvis and ureter - expression in subset of early proximal tubule.
	Nephrogenic zone and cortex - note expression detected in presumptive S1 early proximal tubule adjacent to renal corpuscle.
	Nephrogenic zone and cortex.
	Metanephros sectioned through medulla.
	Nephrogenic zone through to medulla (left). Expression in immature loop of Henle subset.
	Nephrogenic zone and cortex.
	Cortex and medulla (right). Expression in presumptive proximal straight tubule and subset of adjacent immature loop of Henle.
	Metanephros sectioned through pelvis.
	Metanephros sectioned through pelvis.
	Cortex through to pelvis (lower left) - expression in subset of immature loop of Henle.
	Cortex and medulla (top) - expression in subset of immature loop of Henle adjacent to the proximal tubule, presumptive S3 straight segment.
	Metanephros sectioned through pelvis.
	Cortex - note expression in presumptive S1 segment of early proximal tubule adjacent to renal corpuscles of some but not all nephrons (centre). This may reflect the maturation of the different nephrons.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Sean Grimmond, Dave Tang, Rathi Thiagarajan, Kylie Georgas, Melissa Little, Han Sheng Chiu, Divya Ramanth, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	214
Submission ID:	9999991556

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Fbp2

Name: fructose bisphosphatase 2 (MGI:95491)

Probe ID:

MGI:3571926 (maprobe:6042)

Name of cDNA:

RIKEN clone I920078I07

Sequence ID:

AK146946.1

5' primer sequence:

CCCTCTGGATGGATCTTCAA

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCTGTGGTTGCCATACCTCCT

5' primer location:

483

3' primer location:

1021

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

3020

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

10 metanephros and 2 ureter sections examined. Experiment: SISH101109 Probe ID 3020. Imaging: Batch scan 17-11-09 Image_16.vsi. Colour developed for 70hrs. Control probes Wnt4, Wnt7b and Shh colour developed for 47, 47 and 70 hrs respectively. Shh expression was not detected therefore low levels of mRNA expression will not be detected for SISH performed in this experiment. Gene identified as early proximal tubule specific via microarray analysis of Brunskill et al, 2009 microarray data performed by Rathi Thiagarajan.

Linked Publications

Linked Submissions

Acknowledgements