Expression Database

GUDMAP:11371

Data Source

Gene

Dll1

delta-like 1 (Drosophila)

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros sectioned through pelvis.
	Nephrogenic zone and cortex (top) - note expression in medial segment of S-shaped body.
	Pelvis (top) and medulla.
	NZ and cortex - moderate expression in pretubular aggregate (left), strong expression renal vesicle (top right).
	Metanephros sectioned through pelvis.
	NZ and cortex - note expression in upper limb of comma-shaped body (lower left).
	NZ and cortex - expression in upper comma-shaped body, distal renal vesicle, medial S-shaped body and cortical renal tubule of capillary loop nephron.
	NZ and cortex.
	NZ and cortex - note expression in anlage of loop of Henle of capillary loop nephron (centre).
	NZ and cortex - note expression in distal renal vesicles.
	NZ and cortex - note expression in distal renal vesicle and regional expression in a small segment of the cortical renal tubules of capillary loop stage nephron (centre top).
	Metanephros sectioned through ureter.
	Proximal ureter.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Bruce Aronow, Dave Tang, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe, Melissa Little
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	158
Submission ID:	99999995

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Dll1

Name: delta-like 1 (Drosophila) (MGI:104659)

Probe ID:

maprobe:5538

Name of cDNA:

1200004G03

Sequence ID:

NM_007865.3

5' primer sequence:

CTAGAACACTCTGGGAGCGG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTCTTCAAAGACCCAGGGATG

5' primer location:

3' primer location:

535

Template Sequence:

(Click to see sequence.)

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2246

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7µm sections 21 sections examined including metanephros and proximal ureter. Experiment: SISH100908 Dll1 2246 Slide A5. Imaging: Batch Scan 190908 Image_48.vsi. Bruce Aronow and Melissa Little analysis of developing 15.5dpc kidney microarray data (Brunskill et al 2008) - Putative distal renal vesicle subcompartment.

Linked Publications

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:11372	same experiment same probe same specimen

Acknowledgements