Expression Database

GUDMAP:10716

Gene:	Sox10, SRY (sex determining region Y)-box 10
Stage:	Theiler Stage 22
Tissue:	urinary system,male reproductive system
Species:	Mus musculus
Assay Type:	TG wholemount

Images

Mag: 70X. Whole mount urogenital tract, ventral global view. Expression is present at the top aspect of the image in the adrenal medulla. Single cells migrating from the adrenal glands, around the metanephros, enter the rostral portion of the mesonephros. Single cells enter the metanephros and pelvis from the sympathoadrenal/preaortic celiac and mesenteric ganglia (bright area between metanephros). Streams of cells within the gonadal nerve extend from the sympathoadrenal precursors around the caudal portion of the metanephros toward the testis in chains. Transgene expressing cells are visible within the hypogastric nerve beginning on the dorsal side of the urogenital system and wraps laterally around the bladder. Expression in the testis is restricted to the primary sex cords. Intense clusters of cells within pelvic ganglia at the dorsal aspect of the bladder are faintly visible on the left side of the bladder. Expression in the genital tubercle is restricted to lines of cells adjacent to the caudal midline extending toward the distal tip where they encircle the forming glans. Single cell regional expression in the dorsal portion of the preputial swelling is evident.

Submitters

Principal Investigator(s):	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Contributors:	Sara J Ireland, V Ashley Cantrell, E Michelle Southard-Smith
Submitted By:	E Michelle Southard-Smith, Vanderbilt University Medical Centre, Nashville, TN, United States, Michelle.Southard-Smith@vanderbilt.edu
Batch ID:	122
Submission ID:	MS2_010208_4

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

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Specimen

Stage:

TS22

Other Staging System:

14.5 dpc

Tissue:

urinary system,male reproductive system

Strain:

C3HeB/FeJ

Genotype:

Non-wild type Allele:
Gene		Sox10
MGI ID		MGI:3769269
MGI Symbol or lab name		Tg(Sox10-HIST2H2BE/Venus)ASout
Type		Transgenic (Reporter)
Allele Second Chromatid		wild type
Notes:		PMID:21504042

Sex:

male

Specimen Preparation:

wholemount

	Fixation Method:	Neutral buffered formalin
	Embedding:	Unspecified

Experiment Notes:

Specimens fixed for 2-4 hours in NBF

Transgene Visualisation

Reporter:	H2BVenus
Direct method for visualising reporter:	transgene fluorescence