Expression Database

GUDMAP:9974

Data Source

Gene

Igf1	insulin-like growth factor 1

Theiler Stage

TS21

Tissue

urinary system

Images

	Metanephroi
	Global view of specimen dorsal side
	Global view of specimen ventral side

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Kylie Georgas, Melissa Little, Darrin Taylor, Bree Rumballe, Han Sheng Chiu
Submitted By:	Han Sheng Chiu, University of Queensland, Brisbane, Australia, h.chiu@imb.uq.edu.au
Archive ID:	121
Submission ID:	ml_240608_7419

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Igf1

Name: insulin-like growth factor 1 (MGI:96432)

Probe ID:

MGI:2413015 (maprobe:4954)

Name of cDNA:

RIKEN clone B930068C16

Sequence ID:

AK081019.1

5' primer sequence:

TTTTCGCCTCATTATCCCTG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTGTTTTGCAGGTTGCTCAAG

5' primer location:

226

3' primer location:

818

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

1816

Specimen

Theiler Stage:

TS21

Other Staging System:

13.5dpc

Tissue:

urinary system

Strain:

CD1

Genotype:

Wild type

Sex:

male

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Male urogenital tract removed and upper urinary tract examined. 1 specimen examined. Male and female upper urinary tracts were examined only the male is shown. Male expression pattern in the metanephros is identical to female. Pelvic messenchyme and MM (Potter Structural) list. Experiment: WISH250907 PROBE ID 1816 Colour development for 100min. Control probe Wnt4 colour developed for 110min on TS21 whole urogenital tract.