Expression Database

GUDMAP:9904

Data Source

Gene

Upk3a

uroplakin 3A

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros and proximal ureter
	Nephrogenic zone and cortex and medulla-expression is absent from the cortical collecting and detected in the medullary collecting duct
	Medulla and pelvis-expression in medullary collecting duct and pelvic urothelial lining
	Metanephros
	Medulla-strong expression in collecting duct.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Darrin Taylor, Han Sheng Chiu, Bree Rumballe, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Kylie Georgas
Submitted By:	Bree Rumballe, University of Queensland, Brisbane, Australia, b.rumballe@imb.uq.edu.au
Archive ID:	118
Submission ID:	ml_010508_7271

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Upk3a

Name: uroplakin 3A (MGI:98914)

Probe ID:

MGI:1900588 (maprobe:4943)

Name of cDNA:

RIKEN clone 1110017C07

Sequence ID:

AK003737.1

5' primer sequence:

AGCGGCCACTGAGTACAGAT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTTCATGAGGTTGGACCAAGA

5' primer location:

3' primer location:

579

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM Purple

Lab Probe ID:

1872

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7µm sections 12 sections examined including metanephros and proximal ureter. Experiment: SISH050907 1872 Upk3a Robot SISH Slide B5 Colour development for24hrs. Control probes Wnt4, Wnt7b and Shh on TS23 metanephros colour developed for 24hrs, 24hrs and 98hrs respectively. Imaging: Batch Scan 110907 Image_17.vsi. UQ subcompartment Method1 - Medullary UB

Linked Publications

Linked Submissions

Acknowledgements