Expression Database

GUDMAP:9817

Data Source

Gene

Ppp1r3c

protein phosphatase 1, regulatory (inhibitor) subunit 3C

Theiler Stage

TS21

Tissue

urinary system

Images

	Metanephroi
	Global view of specimen dorsal side

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Emmanuelle Lesieur, University of Queensland, Brisbane, Australia, e.lesieur@imb.uq.edu.au
Archive ID:	117
Submission ID:	ml_300408_7184

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Ppp1r3c

Name: protein phosphatase 1, regulatory (inhibitor) subunit 3C (MGI:1858229)

Probe ID:

MGI:2396729 (maprobe:4925)

Name of cDNA:

RIKEN clone 6820424F13

Sequence ID:

AK078506.1

5' primer sequence:

TTGAAGCACATCTGGCTTTG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGACAATCCACTGCACTTTCCC

5' primer location:

1471

3' primer location:

2107

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

1894

Specimen

Theiler Stage:

TS21

Other Staging System:

13.5dpc

Tissue:

urinary system

Strain:

CD1

Genotype:

Wild type

Sex:

male

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Male urogenital tract removed and upper urinary tract examined. 2 specimens examined. Male and female upper urinary tracts were examined only the male is shown. Male expression pattern in the metanephros is identical to female. Experiment: WISH 271107 PROBE 1894 Colour development for 120min. Control probe Wnt4 colour developed for 150min on TS21 metanephric explant

Linked Publications

Linked Submissions

Acknowledgements