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Expression Database
GUDMAP:9680
Data Source
Gene
Olfml3
olfactomedin-like 3
Theiler Stage
TS21
Tissue
urinary system
Images
Metanephroi
Global view of specimen dorsal side
Global view of specimen ventral side
Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (
more info
).
Submitters
Principal Investigator(s):
Melissa H Little,
University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:
Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe, Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas
Submitted By:
Han Sheng Chiu,
University of Queensland, Brisbane, Australia, h.chiu@imb.uq.edu.au
Archive ID:
115
Submission ID:
ml_140408_7051
Expression Mapping
Expression Strengths Key:
Present (unspecified strength)
Present (strong)
Present (moderate)
Present (weak)
Uncertain
Not Detected
Expression Patterns Key:
Homogeneous
Graded
Regional
Spotted
Ubiquitous
Restricted
Single cell
Contains note
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Probe
Gene:
Symbol:
Olfml3
Name:
olfactomedin-like 3
(
MGI:1914877
)
Probe ID:
MGI:1896775
(maprobe:4908)
Name of cDNA:
RIKEN clone 0610011B12
Sequence ID:
AK002518.1
5' primer sequence:
CTGCAGAGAGCCTGATACCC
3' primer sequence:
CGATGTTAATACGACTCACTATAGGGAGGGGCTTGAGAATTTGGTT
5' primer location:
802
3' primer location:
1393
Origin of Clone used to make the Probe:
Strain:
Tissue:
Probe Type:
RNA
Type:
antisense
Labelled with:
digoxigenin
Visualisation method:
alkaline phosphatase + NBT/BCIP
Lab Probe ID:
1811
Specimen
Theiler Stage:
TS21
Other Staging System:
13.5dpc
Tissue:
urinary system
Strain:
CD1
Genotype:
Wild type
Sex:
male
Specimen Preparation:
wholemount
Fixation Method:
4% paraformaldehyde
Experiment Notes:
Male urogenital tract removed and upper urinary tract examined. 1 specimen examined. Male and female upper urinary tracts were examined only the male is shown. Male expression pattern in the metanephros is identical to female. Experiment: WISH091007 PROBE ID 1811.Pelvic mesenchyme and MM set. Colour development for 160min. Control probe Wnt4 colour developed for 60min on TS21 whole urogenital tract.