Expression Database

GUDMAP:9680
Data Source
Gene
Olfml3 olfactomedin-like 3
Theiler Stage TS21
Tissue urinary system
Images
Metanephroi
Global view of specimen dorsal side
Global view of specimen ventral side
Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).
Submitters
Principal Investigator(s): Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors: Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe, Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas
Submitted By: Han Sheng Chiu, University of Queensland, Brisbane, Australia, h.chiu@imb.uq.edu.au
Archive ID: 115
Submission ID: ml_140408_7051
Expression Mapping

Expression Strengths Key:
Present (unspecified strength)
Present (strong)
Present (moderate)
Present (weak)
Uncertain
Not Detected
   
Expression Patterns Key:
Homogeneous
Graded
Regional
Spotted
Ubiquitous
Restricted
Single cell
Contains note
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Probe
Gene:
Symbol: Olfml3
Name: olfactomedin-like 3 (MGI:1914877)
Probe ID: MGI:1896775 (maprobe:4908)
Name of cDNA: RIKEN clone 0610011B12
Sequence ID:
AK002518.1
5' primer sequence: CTGCAGAGAGCCTGATACCC
3' primer sequence: CGATGTTAATACGACTCACTATAGGGAGGGGCTTGAGAATTTGGTT
5' primer location: 802
3' primer location: 1393
Origin of Clone used to make the Probe:
Strain:
  Tissue:
Probe Type: RNA
Type: antisense
Labelled with: digoxigenin
Visualisation method: alkaline phosphatase + NBT/BCIP
Lab Probe ID: 1811
Specimen
Theiler Stage: TS21
Other Staging System: 13.5dpc
Tissue: urinary system
Strain: CD1
Genotype: Wild type
Sex: male
Specimen Preparation:
wholemount
Fixation Method: 4% paraformaldehyde
Experiment Notes:
Male urogenital tract removed and upper urinary tract examined. 1 specimen examined. Male and female upper urinary tracts were examined only the male is shown. Male expression pattern in the metanephros is identical to female. Experiment: WISH091007 PROBE ID 1811.Pelvic mesenchyme and MM set. Colour development for 160min. Control probe Wnt4 colour developed for 60min on TS21 whole urogenital tract.

 

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Department of Health and Human Services National Institutes of Health Contacts Citing GUDMAP National Institute of Diabetes and National Institute of Child Health and Human Development