Quick search:
Gene
Anatomy
Accession ID
Function
for
Login
Feedback
Home
About GUDMAP
Goals
Projects
Publications
Contacts
Positions
Funding
Events
Links
Message Board
Citing GUDMAP
Gene Expression
Query/Browse Database
Tissue Summaries
ISH Quality Control
Resources
Submission Archive
Project Protocols
Mice/Cell Lines
3D Atlas
Ontology
UQ GUDMAP - Probe Design
Web Stats
Internal
Tutorials
Overview
Urinary Development
Reproductive Development
Disease
Help
Using Database
Website
Glossary
Clinicians
Biologists
Bioinformaticians
Public
System Requirements
Expression Database
Tissue Summaries
Analysis
Annotate
Downloads
Data Source
Collections
Expression Database
Data Source
Gene
Tgfbi
transforming growth factor, beta induced
Theiler Stage
TS21
Tissue
male reproductive system
Images
Testis mesonephros ventral side
Testis mesonephros dorsal side
Global view of specimen ventral side
Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (
more info
).
Submitters
Principal Investigator(s):
Melissa H Little,
University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:
Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:
Han Sheng Chiu,
University of Queensland, Brisbane, Australia, h.chiu@imb.uq.edu.au
Archive ID:
115
Submission ID:
ml_140408_7035
Expression Mapping
Expression Strengths Key:
Present (unspecified strength)
Present (strong)
Present (moderate)
Present (weak)
Uncertain
Not Detected
Expression Patterns Key:
Homogeneous
Graded
Regional
Spotted
Ubiquitous
Restricted
Single cell
Contains note
View annotated components as a list
Show annotation under groups
Javascript Tree Menu
A tree of annotated anatomical components will open here if you enable JavaScript in your browser.
 
 
Probe
Gene:
Symbol:
Tgfbi
Name:
transforming growth factor, beta induced
(
MGI:99959
)
Probe ID:
MGI:2420908
(maprobe:4338)
Name of cDNA:
RIKEN clone D230045E03
Sequence ID:
AK084431.1
5' primer sequence:
GGATGTCCTGAAGGGAGACA
3' primer sequence:
CGATGTTAATACGACTCACTATAGGGTCGGTCTTCCTGCTAATGCT
5' primer location:
1580
3' primer location:
2126
Origin of Clone used to make the Probe:
Strain:
Tissue:
Probe Type:
RNA
Type:
antisense
Labelled with:
digoxigenin
Visualisation method:
alkaline phosphatase + NBT/BCIP
Lab Probe ID:
1808
 
 
Specimen
Theiler Stage:
TS21
Other Staging System:
13.5dpc
Tissue:
male reproductive system
Strain:
CD1
Genotype:
Wild type
Sex:
male
Specimen Preparation:
wholemount
Fixation Method:
4% paraformaldehyde
Experiment Notes:
Male urogenital tract removed and reproductive tissues examined. 1 specimen examined. Experiment: WISH091007 PROBE ID 1808.Pelvic mesenchyme and MM set. Colour development for 100min. Control probe Wnt4 colour developed for 60min on TS21 whole urogenital tract.
Linked Publications
Linked Submissions
Acknowledgements