Expression Database

Data Source

Gene

pleiotrophin

Theiler Stage

Images

	Image at 4X magnification. Development time for this sample was 18hrs. Note the surface vasculature labeled throughout the urogenital system.
	Image at 8X magnification.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	M Todd Valerius, Andrew P McMahon
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	111
Submission ID:	021224oWMtv_4

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Ptn

Name: pleiotrophin (MGI:97804)

Probe ID:

maprobe:4866

Name of cDNA:

UI-M-AI0-aal-g-07-0-UI

Additional Name of cDNA:

TUB5004

Sequence ID:

AI836517

Origin of Clone used to make the Probe:

		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

UI-M-AI0-aal-g-07-0-UI

Probe Notes:

Probe template generated by PCR using T3 and T7 primers to amplify clone insert (vector = pT7T3D-Pac). Clone from BMAP-UG library. Clone ID= UI-M-AQ0-aag-b-10-0-UI. Polymerase used to generate antisense ribroprobe = T3. TUB5002.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Linked Publications

Linked Submissions

Acknowledgements


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