Expression Database

GUDMAP:8849

Data Source

Gene

Prickle2

prickle homolog 2 (Drosophila)

Theiler Stage

TS17

Tissue

genitourinary system

Images

	Global view of specimen rostral end on left.
	Metanephric mesenchyme and caudal mesonephros.
	Rostral mesonephros - signal likely to be absent from the mesonephric tubules.
	Global view of specimen 2 rostral end on left.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Sean Grimmond, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Melissa Little, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	91
Submission ID:	ml_251007_6524

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Prickle2

Name: prickle homolog 2 (Drosophila) (MGI:1925144)

Probe ID:

MGI:1909581 (maprobe:4701)

Name of cDNA:

RIKEN clone 6230400G14

Sequence ID:

AK018075.1

5' primer sequence:

CTCCATTTCCTCTTCCTCCC

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGCCATACACACATACGCTGG

5' primer location:

3' primer location:

560

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

1789

Specimen

Theiler Stage:

TS17

Other Staging System:

Tissue:

genitourinary system

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Embryo dissected transversely below the forelimb and sagittally bisected down the midline and the organs removed to reveal the urogenital tract. The hindlimb is visible and the rostral end of the embryo is on the left. 3 specimens examined. WISH110907 1789 Colour development for 1260min. Control probes Wnt4 and Shh colour developed for 90min and 1260min (21hr) respectively on TS20 whole urogenital tract.