Expression Database

Data Source

Gene

guanine nucleotide binding protein (G protein), beta 5

Theiler Stage

Images

	Magnification = 3X. Development time for this sample = 12hrs.
	Magnification = 7X. Development time for this sample = 12hrs.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	Andrew P McMahon, M Todd Valerius, Jing Yu
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	84
Submission ID:	MCM_31102007_089

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Gnb5

Name: guanine nucleotide binding protein (G protein), beta 5 (MGI:101848)

Probe ID:

maprobe:4588

Name of cDNA:

0610030J18

Sequence ID:

AK018747

5' primer sequence:

CACAGGATGGGAAGGTGATTGTCTG

3' primer sequence:

TAATACGACTCACTATAGGGTGCTCTCCTTGGAGTAGATG

5' primer location:

3' primer location:

602

Origin of Clone used to make the Probe:

cDNA		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

MSR0609

Probe Notes:

Probe template was amplified from the RIKEN clone 0610030J18 using primers desinged to the coding region. A T7 promoter sequence was added to the reverse primer for transcription. The primers used are as follows: Reverse primer name= Gnb5-RIK-AK018747-reverse T7, length=40nt, sequence= TAATACGACTCACTATAGGGTGCTCTCCTTGGAGTAGATG ; Forward primer name= Gnb5-RIK-AK018747-forward, length=25nt, sequence= CACAGGATGGGAAGGTGATTGTCTG This probe is designed to detect the gene identified by geneID= 14697

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

male

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Linked Publications

Linked Submissions

Acknowledgements

Mary Duah, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project.