Expression Database

GUDMAP:8311

Data Source

Gene

Cyp51

cytochrome P450, family 51

Theiler Stage

TS20

Images

	Primitive bladder ventral side. Signal can be seen in the epithelium in the centre of the bladder.
	Global view of specimen ventral side.
	Global view of specimen dorsal side.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Han Sheng Chiu, Kylie Georgas, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	86
Submission ID:	ml_0308_6409

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

View annotated components as a list

Show annotation under groups

Javascript Tree Menu

Probe

Gene:

Symbol: Cyp51

Name: cytochrome P450, family 51 (MGI:106040)

Probe ID:

MGI:2390819 (maprobe:4462)

Name of cDNA:

RIKEN clone 4732458H05

Sequence ID:

AK028815.1

5' primer sequence:

GTCGTGTAGTTGGGCCACTT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTGGCCTCAAATTCTCAATCC

5' primer location:

2030

3' primer location:

2616

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

1485

Specimen

Theiler Stage:

TS20

Other Staging System:

Tissue:

Strain:

Quackenbush

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Female urogenital tract removed and lower urinary tract examined. 1 specimen examined. WISH060307 1485 Colour development for 180min. Control probes Wnt4 and Shh colour developed for 100min and 250min respectively on TS20 whole urogenital tract.