Expression Database

GUDMAP:8210

Data Source

Gene

Ptn	pleiotrophin

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephroi. Wt1 antibody visualised with DAB in orange.
	Metanephros
	Cortex maturing renal corpuscles show Wt1 in podocytes and Ptn in mesangium and S-shaped body
	Cortex capillary loop and maturing nephrons Ptn signal in early tubule most likely distal tubule
	Cortex and nephrogenic zone Ptn overlaps with Wt1 in cap mesenchyme and nephrogenic interstitium. Ptn also in ureteric tip.
	Cortex and nephrogenic zone Ptn weak or absent from renal vesicles and pretubular aggregates. Ptn is strong in the interstitium adjacent to these structures.
	Cortex shows Ptn absent from comma-shaped body and on distal segment of S-shaped body with weaker expression in proximal S-shaped body
	Comma shaped body with weak expression adjacent to lower limb where Wt1 is expressed

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Kylie Georgas, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	81
Submission ID:	ml_280507_6242

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Ptn

Name: pleiotrophin (MGI:97804)

Probe ID:

MGI:1898326 (maprobe:4198)

Name of cDNA:

RIKEN clone 2610008G07

Sequence ID:

AK011346.1

5' primer sequence:

GCACAGCCTGCTTCTTCTCT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTTCAAGGCGGTATTGAGGTC

5' primer location:

3' primer location:

674

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

1379

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

14 metanephros 7µm sections including proximal ureter. RNA in situ hybridisation with Ptn antisense riboprobe visualised with NBT/BCIP which produces blue signal then over the top is immunohistochemistry with anti-Wt1 antibody visualised with DAB which produces orange signal. The details of the antibody used can be found in the linked submissions. Experiment: IHC110107 Imaging: Batchscan110107_5.vsi

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:7650	same probe