Expression Database

GUDMAP:8018

Data Source

Gene

Paics

phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase

Theiler Stage

TS17

Images

	Global view specimen rostral end on left
	Metanephric mesenchyme and caudal mesonephros
	Rostral mesonephros

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Han Sheng Chiu, Kylie Georgas, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	75
Submission ID:	ml_090507_6188

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Paics

Name: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase (MGI:1914304)

Probe ID:

MGI:1895253 (maprobe:4364)

Name of cDNA:

RIKEN clone 2410011K06

Sequence ID:

AK010462.1

5' primer sequence:

GAGTCCTCCTGCAGTCCAAG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGTTTGTCCTTCTGCTGGCTC

5' primer location:

107

3' primer location:

703

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

1467

Specimen

Theiler Stage:

TS17

Other Staging System:

Tissue:

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Embryo dissected transversly below the forelimb and saggitally bissected down the midline and the organs removed to reveal the urogenital tract. The hindlimb is visible and the rostral end of the embryo is on the left. 3 samples examined. WISH060207 1467 Colour development for 40min. Control probes Shh and Wnt4 colour developed for 21hrs20min and 130min respectively.

Linked Publications

Linked Submissions

Acknowledgements