Expression Database

Data Source

Gene

Ptn

pleiotrophin

Theiler Stage

TS23

Tissue

metanephros

Images

	Metanephros.
	Magnification 1st image signal in renal tubule possibly immature loop of Henle as this is likely to be renal medulla.
	Pelvis signal in perihilar interstitium and pelvic smooth muscle.
	Cortex: note signal in maturing renal corpuscle glomerular mesangium and cortical renal tubule subset posssibly early distal tubule.
	Metanephros.
	Cortex.
	Cortex. Strongest signal in renal cortical interstitium closest to nephrogenic zone and glomerular mesangium of renal corpuscles.
	Metanephros. Some ureteric tips show expression

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Kylie Georgas
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	60
Submission ID:	uq_010207_5760

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

View annotated components as a list

Show annotation under groups

Javascript Tree Menu

Probe

Gene:

Symbol: Ptn

Name: pleiotrophin (MGI:97804)

Probe ID:

maprobe:4351

Name of cDNA:

Sequence ID:

AK011346.1

5' primer sequence:

GCACAGCCTGCTTCTTCTCT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTTCAAGGCGGTATTGAGGTC

5' primer location:

3' primer location:

674

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

1379

Specimen

Theiler Stage:

TS23

Other Staging System:

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

Fixation Method:

4% paraformaldehyde

Experiment Notes:

14 metanephros 7um sections including proximal ureter. RNA in situ hybridisation with Ptn antisense riboprobe visualised with NBT/BCIP which produces blue signal then over the top is immunohistochemistry with anti-Wt1 antibody by Dak°Cytomation catalogue number 6F-H2 and visualised with DAB which produces orange signal. IHC110107 Batchscan110107_5.vsi

Linked Publications

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:8210	same probe

Acknowledgements