Expression Database

GUDMAP:7504

Data Source

Gene

P4ha2

procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide

Theiler Stage

TS17

Tissue

genitourinary system

Images

	Global view specimen 1 rostral end on left
	Global view specimen 2 rostral end on left

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Kylie Georgas
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	59
Submission ID:	uq_310107_5648

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: P4ha2

Name: procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide (MGI:894286)

Probe ID:

MGI:2425295 (maprobe:4273)

Name of cDNA:

RIKEN clone E130103J11

Sequence ID:

AK053504.1

5' primer sequence:

CCAAGCTCGCCAAGATTAAG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTACAAGTTTTCCTGGGTGCC

5' primer location:

388

3' primer location:

1060

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1391

Specimen

Theiler Stage:

TS17

Other Staging System:

Tissue:

genitourinary system

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Embryos were sagitally dissected below the forelimb and hindlimb and bissected down the midline to expose the urogenital system. The hindlimb bud is visible and the caudal end is on the right. 3 specimens examined. WMISH241006 1391. Colour development for 2hrs.

Linked Publications

Linked Submissions

Acknowledgements