Expression Database

GUDMAP:6951

Gene

Exoc3l2 exocyst complex component 3-like 2

Theiler Stage

Images



	Tunica albuginea and rete testis area are strongly positive.
	Tunica albuginea and rete testis area are strongly positive.
	Tunica albuginea area is strongly positive.

Submitters

Principal Investigator:	Kevin Gaido, The Hamner Institutes of Health Sciences, 6 Davis Dr, Research Triangle Park, gaido@thehamner.org
Authors:	Helen Shi, Kamin Johnson, Kevin Gaido
Submitted By:	Janan Hensley, The Hamner Institutes of Health Sciences, 6 Davis Dr, Research Triangle Park, jhensley@thehamner.org
Archive ID:	39

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Probe Name:

MGI:1907640

Clone Name:

RIKEN clone 4933417E01

Sequence:

Fully Sequenced. Probe sequence spans from 707 to 1236 of AK016842.1

Gene:

Symbol: Exoc3l2

Name: exocyst complex component 3-like 2 (MGI:1921713)

5' primer sequence:

TGCTGCTGATAGACCCTCCT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTGGCTCAGGACAGACAAGTG

5' primer location:

707

3' primer location:

1236

Curator Notes:

Clone name changed from RIKEN clone 4933417E01 to Riken Clone 4933417E01

Genbank accession changed from NM_028954.1 to AK016842.1

5 prime location changed from 1165 to 707

3 prime location changed from 1867 to 1236

5 prime sequence changed from ACTCCTCAGTTGCCGTTGAT to TGCTGCTGATAGACCCTCCT

3 prime sequence changed from AAGTGCCCACAGTCAGTTCC to CGATGTTAATACGACTCACTATAGGGTGGCTCAGGACAGACAAGTG

Symbol changed from: 4933417E01Rik to: Exoc3l2

Origin of Clone used to make the Probe:

cDNA		Strain:
		Tissue:	testis

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Author Notes:

The template DNA sequence was PCR amplified from pool of various gestation ages of CD1 mouse testis cDNA using: 5' primer: ACTCCTCAGTTGCCGTTGAT, 3' primer: AAGTGCCCACAGTCAGTTCC. T7 is incorporated in the transcription step using DIG RNA Labeling kit.

Specimen

Theiler Stage:

TS24

Other Staging System:

Strain:

CD1

Sex:

male

Genotype:

Wild Type

Specimen Preparation:

Section

	Fixation Method:	4% paraformaldehyde
	Embedding:	cryosection

Experiment Notes:

Linked Publications

Linked Submissions

Acknowledgements