Expression Database

GUDMAP:6842

Data Source

Gene

Il1rapl1

interleukin 1 receptor accessory protein-like 1

Theiler Stage

TS23

Images

	Female, 3x magnification. Development time = 48 hours.
	Female, 7x magnification. Development time = 48 hours.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	Jing Yu, Andrew P McMahon, M Todd Valerius
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	30
Submission ID:

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Il1rapl1

Name: interleukin 1 receptor accessory protein-like 1 (MGI:2687319)

Probe ID:

maprobe:4116

Name of cDNA:

UI-M-BG1-aii-f-07-0-UI

Additional Name of cDNA:

MSR0883

Sequence ID:

AI850804

Origin of Clone used to make the Probe:

		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Probe Notes:

Probe template generated by PCR using T3 and T7 primers to amplify clone insert (vector = pT7T3D-Pac). Clone from BMAP-UG library. Clone ID= UI-M-BG1-aii-f-07-0-UI. Orientation checked by sequencing. Polymerase used to generate antisense riboprobe = T7. MSR0883

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

Female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Acknowledgements

Jenn Hansard and Mary Duah are acknowledged for their technical assistance on the GUDMAP project.