Expression Database

GUDMAP:6770

Data Source

Gene

Adra1b

adrenergic receptor, alpha 1b

Theiler Stage

TS23

Images

Female, 3x magnification. Development time = 48 hours.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	Andrew P McMahon, M Todd Valerius, Jing Yu
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	30
Submission ID:

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Adra1b

Name: adrenergic receptor, alpha 1b (MGI:104774)

Probe ID:

maprobe:4080

Name of cDNA:

UI-M-AO0-acf-f-11-0-UI

Additional Name of cDNA:

MSR0086

Sequence ID:

AI838767

Origin of Clone used to make the Probe:

		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Probe Notes:

Probe template generated by PCR using T3 and T7 primers to amplify clone insert (vector = pT7T3D-Pac). Clone from BMAP-UG library. Clone ID= UI-M-AO0-acf-f-11-0-UI. Orientation checked by sequencing. Polymerase used to generate antisense riboprobe = T3. (MSR0086)

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

Female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Linked Publications

Linked Submissions

Acknowledgements

Jenn Hansard and Mary Duah are acknowledged for their technical assistance on the GUDMAP project.