Expression Database

Data Source

Gene

Rho guanine nucleotide exchange factor (GEF) 12

Theiler Stage

Images

	Male, 3X mag.
	Male, 7X mag.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	Andrew P McMahon, M Todd Valerius, Jing Yu
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	10
Submission ID:

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Arhgef12

Name: Rho guanine nucleotide exchange factor (GEF) 12 (MGI:1916882)

Probe ID:

maprobe:3995

Name of cDNA:

UI-M-AH0-act-a-12-0-UI

Additional Name of cDNA:

MSR0132

Sequence ID:

AI840231

Origin of Clone used to make the Probe:

		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Probe Notes:

Probe template generated by PCR using T3 and T7 primers to amplify clone insert (vector = pT7T3D-Pac). Clone from BMAP-UG library. Clone ID= UI-M-AH0-act-a-12-0-UI (MSR0132).

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

male

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

The colour reaction time in BM purple for this probe on this sample was 24 hours.

Linked Publications

Linked Submissions

Acknowledgements

Karl Staser, Jenn Hansard & Mary Duah are acknowledged for their technical assistance on the GUDMAP project.