Expression Database

Data Source

Gene

prolactin family 3, subfamily b, member 1

Theiler Stage

Images

	The caudal end of the embryo is on the right. The proximal hindlimb bud is just visible at the bottom, middle. The nephric duct and ureteric bud (middle) are visible and show no specific expression above background. There is specific expression, above background in the metanephric mesenchyme dorsal to the bud and continues in the mesonephros (the caudal end of which is visible).
	The caudal end of the embryo is on the right, the hindlimb bud is visible. There is specific expression above background along the intermediate mesoderm in the region of the hindlimb bud. Expression seems to be restricted from the rostral mesonephros though.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Gemma Martinez, Kylie Georgas, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	1
Submission ID:

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Prl3b1

Name: prolactin family 3, subfamily b, member 1 (MGI:97607)

Probe ID:

MGI:3062678 (maprobe:3853)

Name of cDNA:

NIA clone H3046B04

Sequence ID:

BG079872.2

Origin of Clone used to make the Probe:

		Strain:	C57BL/6J
		Tissue:	Blastocyst

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Probe Notes:

Riboprobe made from the full length insert of the NIA clone ID H3046B04. Array probe ID: BG079872. Unigene Cluster: Mm37203.

Curator Notes:

Symbol changed from Csh2 to Prl3b1

Specimen

Theiler Stage:

TS17

Other Staging System:

10.5dpc

Tissue:

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

10.5dpc embryos dissected sagitally down the midline and transversely below the forelimb bud and below the hindlimb bud such that the hindlimb bud is visible, and to visualise the aorta-gonad-mesonephros of the intermediate mesoderm.

Linked Publications

Linked Submissions

Acknowledgements