Expression Database

Data Source

Gene

Dsg2

desmoglein 2

Theiler Stage

TS21

Tissue

urinary system

Images

	Primitive Bladder - expression seen in the epithelium layer of the primitive bladder and possibly a portion of the mesenchymal layer.
	Urogenital tract - Global view from the ventral side. Bladder is annotated in this submission. UGT includes gonad/mesonephros, metanephroi, bladder and genital tubercle. Female reproductive system organs are annotated in a separate submission.
	Genital tubercle - ventral view - expression in urethral epithelium.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Cathy Mendelsohn, Dave Tang, Bree Rumballe, Divya Ramanth, Melissa Little, Sean Grimmond, Han Sheng Chiu, Kylie Georgas
Submitted By:	Divya Ramnath, The University of Queensland, Brisbane, Australia, d.ramnath@imb.uq.edu.au
Archive ID:	223
Submission ID:	9999991649

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Dsg2

Name: desmoglein 2 (MGI:1196466)

Probe ID:

MGI:2391392 (maprobe:5986)

Name of cDNA:

RIKEN clone 4832423K21

Sequence ID:

AK029320.1

5' primer sequence:

CATTGACCGACTTCCATCCT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGAAAGCAAAATCCCCACAAA

5' primer location:

2862

3' primer location:

3504

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Lab Probe ID:

2821

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS21

Other Staging System:

13.5dpc

Tissue:

urinary system

Strain:

CD1

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Urogenital tract whole mount - 1 embryo examined. Experiment: WISH081209 Probe ID 2821. Gene identified as a specific to the urothelium layer of bladder in collaboration with Cathy Mendelsohn. Colour development for 250mins. Control probes Wnt4, Myh11 and Shh colour developed for 100 mins.

Linked Publications

Linked Submissions

Acknowledgements