Expression Database

Data Source

Gene

Atp1b1

ATPase, Na+/K+ transporting, beta 1 polypeptide

Theiler Stage

TS23

Tissue

urinary system

Images

	Bladder.
	Bladder layers.
	Lower urinary tract - sagittal section through bladder and external genitalia. External genitalia are annotated in a separate submission.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Sean Grimmond, Dave Tang, Rathi Thiagarajan, Kylie Georgas, Melissa Little, Han Sheng Chiu, Divya Ramanth, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	222
Submission ID:	9999991800

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Atp1b1

Name: ATPase, Na+/K+ transporting, beta 1 polypeptide (MGI:88108)

Probe ID:

MGI:1895468 (maprobe:6063)

Name of cDNA:

RIKEN clone 2410046B18

Sequence ID:

AK010677.1

5' primer sequence:

AGGCAGCTGGAAGAAATTCA

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCAACACTCGGTTGAGCTTGA

5' primer location:

526

3' primer location:

1051

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2842

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

urinary system

Strain:

CD1

Genotype:

Wild type

Sex:

male

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

Lower urinary tract - embryos were dissected below the forelimb buds, internal organs removed except the lower urinary tract and sectioned sagittally. Male and female embryos were examined. Bladder, urethra and external genitalia were examined. This submission examines the male urinary system organs and a separate submission examines the male reproductive system organs. Experiment: SISH160909 Probe ID 2842. Imaging: Batch scan 23-09-09 Image_26.vsi. Colour developed for 26hrs. Female and male LUTs were examined (see related data submissions).

Linked Publications

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:13649	same embryo same experiment same probe
GUDMAP:13651	same experiment same probe
GUDMAP:13652	same experiment same probe

Acknowledgements