Expression Database

GUDMAP:13629

Data Source

Gene

Wnt4	wingless-related MMTV integration site 4

Theiler Stage

TS27

Tissue

bladder

Images

	Bladder - low magnification image.
	Bladder - high magnification image - expression in the urothelium of the bladder.
	Bladder - high magnification image.
	Bladder - high magnification image.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Dave Tang, Bree Rumballe, Divya Ramanth, Melissa Little, Han Sheng Chiu, Kylie Georgas
Submitted By:	Divya Ramnath, The University of Queensland, Brisbane, Australia, d.ramnath@imb.uq.edu.au
Archive ID:	218
Submission ID:	9999991675

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Wnt4

Name: wingless-related MMTV integration site 4 (MGI:98957)

Probe ID:

MGI:1897489 (maprobe:4223)

Name of cDNA:

RIKEN clone 2810012H16

Sequence ID:

AK012727.1

5' primer sequence:

GCGTAGCCTTCTCACAGTCC

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCTTGAGGACCCAAAAACCAA

5' primer location:

653

3' primer location:

1351

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

Wnt4 CTRL

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS27

Other Staging System:

P 2

Tissue:

bladder

Strain:

CD1

Genotype:

Wild type

Sex:

male

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

Bladder sections - 6 sections examined. Experiment: SISH031109 Probe ID Wnt4 Slide ID D8. Colour development for 44hrs. Imaging: Image_33.vsi from Batch Scan 19-11-09.