Expression Database

GUDMAP:13591

Data Source

Gene

Dsg2

desmoglein 2

Theiler Stage

TS27

Tissue

bladder

Images

	Bladder - low magnification image.
	Bladder - high magnification image - no expression seen in the bladder.
	Bladder - low magnification image.
	Bladder- high magnification image.
	Bladder - overview image.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Dave Tang, Bree Rumballe, Divya Ramanth, Melissa Little, Han Sheng Chiu, Kylie Georgas
Submitted By:	Divya Ramnath, The University of Queensland, Brisbane, Australia, d.ramnath@imb.uq.edu.au
Archive ID:	215
Submission ID:	9999991558

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Dsg2

Name: desmoglein 2 (MGI:1196466)

Probe ID:

MGI:2391392 (maprobe:5986)

Name of cDNA:

RIKEN clone 4832423K21

Sequence ID:

AK029320.1

5' primer sequence:

CATTGACCGACTTCCATCCT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGAAAGCAAAATCCCCACAAA

5' primer location:

2862

3' primer location:

3504

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2821

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS27

Other Staging System:

P 2

Tissue:

bladder

Strain:

CD1

Genotype:

Wild type

Sex:

male

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

Bladder - 9 sections examined. Experiment: SISH170210 Probe ID 2821 Slide ID F5. Gene identified as specific to the urothelium layer of the bladder in collaboration with Jim Lessard. Colour development for 48hrs. Control probes Myh11, Wnt4 and Shh colour developed for 21, 48 and 48 hrs respectively. Imaging: Image_02.vsi from Batch Scan 23-02-10.

Linked Publications

Linked Submissions

Acknowledgements