Expression Database

GUDMAP:13546

Data Source

Gene

4921509J17Rik

RIKEN cDNA 4921509J17 gene

Theiler Stage

TS23

Tissue

bladder

Images

	LUT - low magnification image. Genital tubercle (centre left) with adjacent labioscrotal swelling and bladder (centre).
	Bladder - low magnification image.
	Bladder - high magnification image - uncertain if the expression seen in the bladder is real or background.
	LUT - low magnification image. Bladder (top centre) and pelvic urethra (below).
	Bladder - low magnification image.
	Bladder- high magnification image.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Dave Tang, Bree Rumballe, Divya Ramanth, Sean Grimmond, Han Sheng Chiu, Kylie Georgas
Submitted By:	Divya Ramnath, The University of Queensland, Brisbane, Australia, d.ramnath@imb.uq.edu.au
Archive ID:	213
Submission ID:	9999991423

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: 4921509J17Rik

Name: RIKEN cDNA 4921509J17 gene (MGI:1918107)

Probe ID:

MGI:1903322 (maprobe:6023)

Name of cDNA:

RIKEN clone 4921509J17

Sequence ID:

AK014853.1

5' primer sequence:

TTTTGGGCTGGTGTAGCTTT

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCCATATGCAAACATTTTGTGG

5' primer location:

618

3' primer location:

1127

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2793

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

bladder

Strain:

CD1

Genotype:

Wild type

Sex:

female

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

Lower Urinary Tract - LUT sections - 6 sections examined. Experiment: SISH031109 Probe ID 2793 Slide ID C3. Gene identified as specific to the urothelium layer of bladder in collaboration with Jim Lessard. Colour development for 140hrs. Control probes Myh11, Upk3a, Wnt4 and Shh colour developed for 22, 22, 51 and 140 hrs respectively. Imaging: Image_22.vsi from Batch Scan 19-11-09.

Linked Publications

Linked Submissions

Acknowledgements