Expression Database

GUDMAP:13470

Data Source

Gene

Btc	betacellulin, epidermal growth factor family member

Theiler Stage

TS23

Tissue

bladder

Images

	Lower urinary tract - low magnification image. Genital tubercle and labioscrotal swelling (centre left) and bladder and pelvic urethra (top centre). No expresion in pelvic urethra (below bladder) or phallic urethra (within genital tubercle, synonym urethral epithelium / urethral plate). Weak surface expression in surface epithelium of external genitalia and skin surface epithelia of embryo.
	Bladder - low magnification image.
	Lower urinary tract - overview image.
	Lower urinary tract - low magnification image. Lower half of embryo with lower urinary tract retained, sagittal section. Bladder (centre right).
	Bladder - low magnification image.
	Bladder - high magnification image - no expression seen in the bladder.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Dave Tang, Bree Rumballe, Divya Ramanth, Melissa Little, Han Sheng Chiu, Kylie Georgas
Submitted By:	Divya Ramnath, The University of Queensland, Brisbane, Australia, d.ramnath@imb.uq.edu.au
Archive ID:	212
Submission ID:	9999991485

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Btc

Name: betacellulin, epidermal growth factor family member (MGI:99439)

Probe ID:

MGI:2390432 (maprobe:5982)

Name of cDNA:

RIKEN clone 4632406O07

Sequence ID:

AK076272.1

5' primer sequence:

CAACCAAACAAGCAAGCAAA

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGGAACGAAAACCCACCTTTCA

5' primer location:

1211

3' primer location:

1727

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2815

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

bladder

Strain:

CD1

Genotype:

Wild type

Sex:

female

Specimen Preparation:

section

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Lower Urinary Tract - Caudal half embryos with lower urinary tracts intact were sectioned sagittally - sections - 6 sections examined. Experiment: SISH280909 Probe ID 2815 Slide ID C2. Gene identified as specific to the urothelium layer of bladder in collaboration with Jim Lessard. Colour development for 140hrs. Control probes Myh11, Upk3a, Wnt4 and Shh colour developed for 22 ,22, 69 and 140 hrs respectively. Imaging: Image_20.vsi from Batch Scan 15-10-09.