Expression Database

GUDMAP:13376

Data Source

Gene

4930550L24Rik

RIKEN cDNA 4930550L24 gene

Theiler Stage

TS27

Tissue

bladder

Images

	Bladder - low magnification image.
	Bladder - high magnification image - no expression seen in the bladder.
	Bladder - low magnification image.
	Bladder - high magnification image.
	Bladder - overview image.
	Urethra - low magnification image.
	Urethra - high magnification image - no expression seen in the urethra.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Dave Tang, Bree Rumballe, Divya Ramanth, Jim Lessard, Han Sheng Chiu, Kylie Georgas
Submitted By:	Divya Ramnath, The University of Queensland, Brisbane, Australia, d.ramnath@imb.uq.edu.au
Archive ID:	201
Submission ID:	9999991173

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: 4930550L24Rik

Name: RIKEN cDNA 4930550L24 gene (MGI:1922602)

Probe ID:

MGI:1908679 (maprobe:5934)

Name of cDNA:

RIKEN clone 4930550L24

Sequence ID:

AK016090.1

5' primer sequence:

GCTGAAGAGGTAGGCACCTG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTTCTTCTCAGAGGCACGGTT

5' primer location:

192

3' primer location:

710

Origin of Clone used to make the Probe:

		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2459

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS27

Other Staging System:

P 2

Tissue:

bladder

Strain:

CD1

Genotype:

Wild type

Sex:

male

Specimen Preparation:

section

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Bladder and urethra sections - 8 sections examined. Experiment: SISH060509 Probe ID 2459 Slide ID A13. Gene identified as a specific to mesenchyme layer of neonatal bladder in collaboration with Jim Lessard. Colour development for 120hrs. Control probes Myh11 and Shh colour developed for 49 and 120 hrs respectively. Imaging: Image_51.vsi from Batch Scan 20-05-09.

Linked Publications

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:13375	same experiment same probe

Acknowledgements