Expression Database

GUDMAP:13282

Data Source

Gene

Pou5f1

POU domain, class 5, transcription factor 1

Theiler Stage

TS23

Images

	Sex related differences in Pou5f1 gene expression observed. Note signal strong and specific in the testis, absent from the ovary.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	Andrew P McMahon, Mary Duah, M Todd Valerius
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, valerius@bidmc.harvard.edu
Archive ID:	197
Submission ID:	AM_191009_20

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Pou5f1

Name: POU domain, class 5, transcription factor 1 (MGI:101893)

Probe ID:

maprobe:5904

Name of cDNA:

MMM1013-98478834

Sequence ID:

BC068268.1

5' primer sequence:

GGGCTGGGTGGATCCTCGAA

3' primer sequence:

TAATACGACTCACTATAGGGAAGCTGATTGGCGATGTG

5' primer location:

122

3' primer location:

824

Origin of Clone used to make the Probe:

cDNA		Strain:	unspecified
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

867

Probe Notes:

Probe template was amplified from bacterial cDNA clone MMM1013-98478834 using primers designed to the coding region (when annotated). A T7 promoter sequence was added to the reverse primer for probe transcription. The primers used: Reverse primer sequence = TAATACGACTCACTATAGGGAAGCTGATTGGCGATGTG : Forward primer sequence = GGGCTGGGTGGATCCTCGAA. Pou5f1-BC068268-PCRriboprobe

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

male

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

The development time in BM purple for this sample was 12 hours.

Linked Publications

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:13283	same experiment same probe

Acknowledgements

Jinjin Guo, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project. DF performed mouse dissection. Mary Duah was responsible for clone procurement. MD performed template PCR reactions, hybridizations and initial annotation. MT Valerius designed the probes, annotated the expression patterns and managed the data.