Expression Database

GUDMAP:12937

Data Source

Gene

A630001G21Rik

RIKEN cDNA A630001G21 gene

Theiler Stage

TS23

Images

	Weak and ubiquitous signal throughout. Development time: 24hrs.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	M Todd Valerius, Andrew P McMahon, Mary Duah
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	190
Submission ID:	AM_300709_4

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: A630001G21Rik

Name: RIKEN cDNA A630001G21 gene (MGI:2443131)

Probe ID:

maprobe:5764

Name of cDNA:

A630001G21

Sequence ID:

AK041324.1

5' primer sequence:

CACAATTGACGCCCGCCG

3' primer sequence:

TAATACGACTCACTATAGGGACAATCGCACGGATGGAG

5' primer location:

3' primer location:

543

Origin of Clone used to make the Probe:

cDNA		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1049

Probe Notes:

Probe template was amplified from RIKEN clone A630001G21 using primers designed to the coding region (when annotated). A T7 promoter sequence was added to the reverse primer for probe transcription. The primers used: Reverse primer sequence = TAATACGACTCACTATAGGGACAATCGCACGGATGGAG : Forward primer sequence = CACAATTGACGCCCGCCG (A630001G21Rik-AK041324-PCRriboprobe)

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

male

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Acknowledgements

Jinjin Guo, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project. DF performed mouse dissection. Mary Duah was responsible for clone procurement, template PCR reactions, in situ hybridizations and the annotation. MT Valerius designed the probes and managed the data.