Expression Database

Data Source

Gene

chromodomain helicase DNA binding protein 3

Theiler Stage

Images

	Signal in vasulature appears to be associated with glomerulus; urethra patches present.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	Andrew P McMahon, Mary Duah, M Todd Valerius
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	188
Submission ID:	AM_150709_107

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Chd3

Name: chromodomain helicase DNA binding protein 3 (MGI:1344395)

Probe ID:

maprobe:5714

Name of cDNA:

B230201M16

Sequence ID:

AK045449

5' primer sequence:

AAGCTGAAGCGAGGTCGGCC

3' primer sequence:

TAATACGACTCACTATAGGGTTTCGGTACATTACCAAG

5' primer location:

1200

3' primer location:

1900

Origin of Clone used to make the Probe:

cDNA		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1009

Probe Notes:

Probe template was amplified from RIKEN clone B230201M16 using primers designed to the coding region (when annotated). A T7 promoter sequence was added to the reverse primer for probe transcription. The primers used: Reverse primer sequence = TAATACGACTCACTATAGGGTTTCGGTACATTACCAAG : Forward primer sequence = AAGCTGAAGCGAGGTCGGCC

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

The development time in BM purple for this sample was 6 hours.

Linked Publications

Linked Submissions

Acknowledgements

Jinjin Guo, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project. DF performed mouse dissection. Mary Duah was responsible for clone procurement. MD performed template PCR reactions, hybridizations and initial annotation. MT Valerius designed the probes, annotated the expression patterns and managed the data.