Expression Database

Data Source

Gene

polycystic kidney and hepatic disease 1-like 1

Theiler Stage

Images

	Development time: 24hrs. Weak and ubiquitous signal.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	M Todd Valerius, Mary Duah, Andrew P McMahon, Jing Yu
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	179
Submission ID:	AM_290409_112

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Pkhd1l1

Name: polycystic kidney and hepatic disease 1-like 1 (MGI:2183153)

Probe ID:

MGI:3506837 (maprobe:836)

Name of cDNA:

MTF#1332

Sequence ID:

NM_138674.1

5' primer sequence:

GTGTGGTCCTGGCAAGCACACC

3' primer sequence:

GAACAAGCTGTTGGGGAGCCG

5' primer location:

4264

3' primer location:

5144

Template Sequence:

(Click to see sequence.)

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Linked Publications

Gray PA, Fu H, Luo P, Zhao Q, Yu J, Ferrari A, Tenzen T, Yuk DI, Tsung EF, Cai Z, Alberta JA, Cheng LP, Liu Y, Stenman JM, Valerius MT, Billings N, Kim HA, Greenberg ME, McMahon AP, Rowitch DH, Stiles CD, Ma Q. (2004) Mouse brain organization revealed through direct genome-scale TF expression analysis. Science, 306, 2255-7

Linked Submissions

Acknowledgements

Jinjin Guo, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project. JV & DF performed mouse dissection. Mary Duah & JG performed hybridizations. MD & MT Valerius annotated the expression patterns and managed the data.