Expression Database

Data Source

Gene

Tle4

transducin-like enhancer of split 4, homolog of Drosophila E(spl)

Theiler Stage

TS21

Tissue

female reproductive system

Images

	Genital tubercle-ventral view
	Genital tubercle-lateral view
	Genital tubercle-dorsal view
	Global view of specimen ventral side
	Global view of specimen dorsal view

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Sean Grimmond, Dave Tang, Kylie Georgas, Han Sheng Chiu, Jim Lessard, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe, Melissa Little
Submitted By:	Han Sheng Chiu, University of Queensland, Brisbane, Australia, h.chiu@imb.uq.edu.au
Archive ID:	176
Submission ID:	999999384

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Tle4

Name: transducin-like enhancer of split 4, homolog of Drosophila E(spl) (MGI:104633)

Probe ID:

MGI:1902881 (maprobe:5647)

Name of cDNA:

RIKEN clone 5730411M05

Sequence ID:

AK017548.1

5' primer sequence:

GTCATTCCTTTCCTGTCCCA

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTGTGCCAGGGATTTTCTTTC

5' primer location:

863

3' primer location:

1466

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

2318

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS21

Other Staging System:

13.5dpc

Tissue:

female reproductive system

Strain:

CD1

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Experiment Notes:

Whole urogenital tract examined from both male and female embryos for this probe. Experiment: WISH291008 Probe ID 2318. Gene identified from Jim Lessard microarray data - expressed in genital tubercle. Colour development for 180min. Control probe Shh colour developed for 120min on TS21 whole urogenital tract.

Linked Publications

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:11752	same experiment same probe
GUDMAP:11753	same experiment same probe
GUDMAP:11755	same experiment same probe

Acknowledgements