Expression Database

GUDMAP:11557

Data Source

Gene

Tnfsf8

tumor necrosis factor (ligand) superfamily, member 8

Theiler Stage

TS23

Images

	Development time: 24hrs.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	Andrew P McMahon, M Todd Valerius
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	173
Submission ID:	AM_190309_22

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Tnfsf8

Name: tumor necrosis factor (ligand) superfamily, member 8 (MGI:88328)

Probe ID:

maprobe:5589

Name of cDNA:

5830454L09

Additional Name of cDNA:

MSR1671

Sequence ID:

AK030916.1

5' primer sequence:

ATGGAGCCAGGGCTGCAACA

3' primer sequence:

TAATACGACTCACTATAGGGAGAAGACGGATAGCACAT

5' primer location:

227

3' primer location:

926

Origin of Clone used to make the Probe:

		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

758

Probe Notes:

Probe template was amplified from RIKEN clone 5830454L09 using primers designed to the coding region. A T7 promoter sequence was added to the reverse primer for probe transcription. The primers used: Reverse primer sequence = TAATACGACTCACTATAGGGAGAAGACGGATAGCACAT : Forward primer sequence= ATGGAGCCAGGGCTGCAACA. MSR1671.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Acknowledgements

Jinjin Guo, Mary Duah, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project. JV & DF performed mouse dissection. MD was responsible for clone procurement. JG & MD performed template PCR reactions and performed hybridizations. MT Valerius designed the probes, annotated the expression patterns and managed the data.