Expression Database

GUDMAP:11404

Data Source

Gene

C1qbp

complement component 1, q subcomponent binding protein

Theiler Stage

TS23

Tissue

metanephros

Images

	Proximal Ureter.
	Metanephros sectioned through medulla and ureter - strongest expression in cap mesenchyme.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe, Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	166
Submission ID:	999999138

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: C1qbp

Name: complement component 1, q subcomponent binding protein (MGI:1194505)

Probe ID:

maprobe:5557

Name of cDNA:

2410027C17

Sequence ID:

AK010601.1

5' primer sequence:

AACAACAGCATCCCTCCAAC

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGCCAAAGGAATAGCGAATGGA

5' primer location:

414

3' primer location:

1017

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

1430

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

metanephros

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7µm sections 4 sections examined including metanephros and proximal ureter. Experiment: SISH200607 1430 Robot SISH Slide A13 Colour development for 25hrs. Control probes Wnt4, Wnt7b and Shh on TS23 metanephros colour developed for 25hrs, 91hrs and 91hrs respectively. Expression was seen with the low level expression control Shh in this experiment therefore genes with a low level expression are likely to be detected. Imaging: BatchScan 06-07-07 Image_12.vsi.