Expression Database

Data Source

Gene

zinc fingers and homeoboxes 2

Theiler Stage

Images

	Development time = 24hrs.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	M Todd Valerius, Andrew P McMahon
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	153
Submission ID:	AM_071108_31

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Zhx2

Name: zinc fingers and homeoboxes 2 (MGI:2683087)

Probe ID:

maprobe:5465

Name of cDNA:

6230424E06

Sequence ID:

AK031782.1

5' primer sequence:

AAGGAGCGGGCATGCCACA

3' primer sequence:

TAATACGACTCACTATAGGGTTGGTAGTATTCAGCGGG

5' primer location:

612

3' primer location:

1311

Origin of Clone used to make the Probe:

		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Lab Probe ID:

667

Probe Notes:

Probe template was amplified from RIKEN clone 6230424E06 using primers designed to the coding region (when annotated). A T7 promoter sequence was added to the reverse primer for probe transcription. The primers used: Reverse primer sequence = TAATACGACTCACTATAGGGTTGGTAGTATTCAGCGGG : Forward primer sequence= AAGGAGCGGGCATGCCACA.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

female

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Linked Publications

Linked Submissions

Acknowledgements

Jinjin Guo, Mary Duah, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project. JV & DF performed mouse dissection. MD was responsible for clone procurement. JG performed template PCR reactions. MT Valerius produced the probes, performed hybridizations, annotated the expression patterns and managed the data.