Expression Database

Data Source

Gene

Igf1

insulin-like growth factor 1

Theiler Stage

TS23

Tissue

ureter

Images

	Proximal ureter.
	Metanephros sectioned through proximal ureter.

Submitters

Principal Investigator(s):	Melissa H Little, University of Queensland, Brisbane, Australia, m.little@imb.uq.edu.au
Authors:	Melissa Little, Darrin Taylor, Han Sheng Chiu, Kylie Georgas, Sean Grimmond, Rathi Thiagarajan, Emmanuelle Lesieur, Bree Rumballe
Submitted By:	Kylie Georgas, University of Queensland, Brisbane, Australia, k.georgas@imb.uq.edu.au
Archive ID:	135
Submission ID:	ml_160708_7567

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Igf1

Name: insulin-like growth factor 1 (MGI:96432)

Probe ID:

MGI:2413015 (maprobe:4954)

Name of cDNA:

RIKEN clone B930068C16

Sequence ID:

AK081019.1

5' primer sequence:

TTTTCGCCTCATTATCCCTG

3' primer sequence:

CGATGTTAATACGACTCACTATAGGGTGTTTTGCAGGTTGCTCAAG

5' primer location:

226

3' primer location:

818

Origin of Clone used to make the Probe:

		Strain:
		Tissue:

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM Purple

Lab Probe ID:

1816

Probe Notes:

Riboprobe generated from a PCR-generated DNA template. The details of the PCR protocol are in the Project Protocols under the Research menu of the website. Primers are designed to amplify a 3'UTR region of the gene of between 500-700bp. The reverse primer is linked to a T7 Polymerase promoter tag sequence.

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

ureter

Strain:

CD1

Genotype:

Wild type

Sex:

unknown

Specimen Preparation:

section

	Fixation Method:	4% paraformaldehyde
	Embedding:	paraffin

Experiment Notes:

7um sections 12 sections examined including metanephros and proximal ureter. Experiment: SISH190208 1816 Robot SISH Slide A10. Colour development: This gene was colour developed for 30hrs. Control probes Wnt4 and Wnt7b on TS23 metanephros colour developed for 48hrs and 54hrs respectively. Shh weak expressing control probe colour developed for 118hrs however no expression could be detected therefore it is possible that very weak levels of gene expression were not detectable in this in situ experiment. Imaging: Batch Scan 04-03-08 Image_36.vsi. Microarray analysis: Developing kidney subcompartments UQ Method 1 - 15.5dpc perihilar interstitium and 11.5dpc metanephric mesenchyme subcompartments.

Linked Publications

Linked Submissions

Acknowledgements