Expression Database

Data Source

Gene

porcupine homolog (Drosophila)

Theiler Stage

Images

	Weak, ubiquitous signal. Development time = 24hrs.

Whole-mount in situ hybridization is subject to technical limitations that may influence accuracy of the data (more info).

Submitters

Principal Investigator(s):	Andrew P McMahon, Harvard University, Cambridge, MA, USA, amcmahon@med.usc.edu
Authors:	M Todd Valerius, Andrew P McMahon
Submitted By:	M Todd Valerius, Harvard University, Cambridge, MA, USA, tvalerius@mcb.harvard.edu
Archive ID:	125
Submission ID:	20080630-41

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

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Probe

Gene:

Symbol: Porcn

Name: porcupine homolog (Drosophila) (MGI:1890212)

Probe ID:

maprobe:5197

Name of cDNA:

2410004O13

Sequence ID:

AK010405.1

5' primer sequence:

GCTGGTGAGATGCACATG

3' primer sequence:

TAATACGACTCACTATAGGGCGGTAGAAGATCCAGCAT

5' primer location:

283

3' primer location:

985

Origin of Clone used to make the Probe:

cDNA		Strain:	C57BL/6
		Tissue:	brain

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + BM purple

Probe Notes:

Probe template was amplified from RIKEN clone 2410004O13 using primers designed to the coding region (when annotated). A T7 promoter sequence was added to the reverse primer for probe transcription. The primers used: Reverse primer sequence = TAATACGACTCACTATAGGGCGGTAGAAGATCCAGCAT : Forward primer sequence= GCTGGTGAGATGCACATG

Specimen

Theiler Stage:

TS23

Other Staging System:

15.5dpc

Tissue:

Strain:

Swiss Webster

Genotype:

Wild type

Sex:

male

Specimen Preparation:

wholemount

Fixation Method:

4% paraformaldehyde

Linked Publications

Linked Submissions

Acknowledgements

Jinjin Guo, Mary Duah, Joe Vaughan & Diane Faria are acknowledged for their technical assistance on the GUDMAP project. JV & DF performed mouse dissection. MD was responsible for clone procurement. JG performed template PCR reactions. MT Valerius produced the probes, performed hybridizations, annotated the expression patterns and managed the data.