Expression Database

GUDMAP:22381

Gene:	Htr3a, 5-hydroxytryptamine (serotonin) receptor 3A
Stage:	Theiler Stage 22
Tissue:	dorsal root ganglion
Species:	Mus musculus
Assay Type:	TG section

Images

	Red channel shows TRPV4 expression, green channel shows Htr3a-EGFP expression. 200x magnification.
	Green channel shows Htr3a-EGFP expression.
	Red channel shows TRPV4 expression.

Results Notes

Expression of Htr3a-EGFP transgene and Trpv4 is noted in EMAPA:16668 lumbar-sacral dorsal root ganglion. EGFP expression is present, strong, and visible in single neuronal cell bodies of the ganglion, as well as axons. Trpv4 is barely present, if at all, in single neuronal cell bodies of the ganglion at 14dpc.

Submitters

Principal Investigator(s):	E. Michelle Southard-Smith, Vanderbilt University, Nashville, TN, USA, Michelle.Southard-Smith@vanderbilt.edu
Contributors:	K. Elaine Ritter
Submitted By:	Elaine Ritter, Vanderbilt University, Nashville, TN, USA, elaine.ritter@vanderbilt.edu
Batch ID:	554
Submission ID:	22381

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Homogeneous
	Graded
	Regional
	Spotted
	Ubiquitous

	Restricted
	Single cell

Nerve Density:

Relative to Total:

	High
	Medium
	Low

Relative to P0/Adult:

	Increase, large
	Increase, small
	Decrease, large
	Decrease, small

	Contains note

View annotated components as a list

Show annotation under groups

Specimen

Stage:

TS22

Other Staging System:

14 dpc

Tissue:

dorsal root ganglion

Strain:

Swiss Webster

Genotype:

Non-wild type Allele:
Gene		Htr3a
MGI ID		MGI:3846657
MGI Symbol or lab name		Tg(Htr3a-EGFP)DH30Gsat
Type		Transgenic (Reporter)
Allele First Chromatid		Tg(Htr3a-EGFP)DH30Gsat
Allele Second Chromatid		wild type

Sex:

male

Specimen Preparation:

section

	Fixation Method:	Neutral buffered formalin
	Embedding:	Cryosection

Experiment Notes:

20uM sagittal cyrosection of lumbar dorsal root ganglia in 14 dpc Htr3a-EGFP transgenic mouse. Camera settings for Images 1, 2 & 3: LSM 510 Meta Confocal Microscope; Objective: Plan-Apochromat 20x/0.75; Imaged Area Size: 450.0um x 450.0um; Scan Mode: Plane, multi-track, 8 bit; Laser Power for Image 1: 488 - 15%, 543nm - 40%; Laser Power for Image 2: 488 - 15%; Laser Power for Image 3: 543nm - 40%; Pinhole: 94um.

Transgene Visualisation

Reporter:	EGFP
Direct method for visualising reporter:	transgene fluorescence

Linked Submissions

Database: GUDMAP

Accession ID	Link Type(s)
GUDMAP:22382	same section